bacillus megaterium atcc 14581 cultures  (ATCC)

Journal: Nature Communications

Article Title: Cobamide-producing microbes as a model for understanding general nutritional interdependencies in soil food webs

doi: 10.1038/s41467-025-68255-6

Figure Lengend Snippet: a Vitamin B 12 production by Bacillus megaterium ATCC 14581 and Streptomyces violaceus strain NBC_00450 at 10 8 CFU/mL ( n = 3 biological replicates per strain). Statistical significance was assessed using a two-sided t test ( t (4) = 5.568, p = 0.0025, 95% CI = [−1.24, −0.41]). b Length of E. crypticus adults after oral supplementation with B. megaterium ATCC 14581 or the S. violaceus strain NBC_00450 ( n = 10 biological replicates per treatment). Statistical significance was determined using two-sided unpaired t test (between control and treatment). c Hatchability of E. crypticus eggs ( n = 10), d length of E. crypticus larvae ( n = 10), e length of E. crypticus adults ( n = 10), and f ovulation rate ( n = 10) after oral supplementation with B. megaterium ATCC 14581 (10 5 CFU/mL) and Vitamin B 12 (positive control). The blue, brown and green dotted lines indicate the control, B. megaterium ATCC 14581 and Vitamin B 12 treatment groups, respectively. Statistical significance was determined using a two-sided unpaired t -test (between control and treatment). Exact statistical data for panels b-f are provided in Supplementary Data 6. g Vitamin B 12 concentrations detected in Bacillus megaterium ATCC 14581 cultures supplemented with 0, 1, or 10 μM CoCl₂ after 48 h ( n = 10). Data were analyzed by two-sided one-way ANOVA ( F (2, 27) = 25.08, η ² = 0.65, p = 0.0037) with Tukey’s multiple comparison test. h Growth rates of adult E. crypticus on day 7 following oral administration of Bacillus megaterium ATCC 14581 supplemented with different concentrations of CoCl₂ (0, 1, and 10 μM) ( n = 10). Statistical significance was determined using a two-sided one-way ANOVA [ F (2, 27) = 16.31, η ² = 0.88, p < 0.0001] with Tukey’s multiple comparison test. i Oral supplementation with B. megaterium ATCC 14581 significantly enhanced the functions of the gut microbiome, including cellular processes (green), metabolism (red), genetic information processing (blue) and organismal systems (purple). The color scale represents the normalized abundances from high (brown) to low (blue). Data are presented as mean ± s.e.m. from independent biological replicates (each replicate derived from a separate batch of individuals or cultures). No technical replicates were used for statistical inference. Source data are provided as a file.

Article Snippet: Exact statistical data for panels b-f are provided in Supplementary Data 6. g Vitamin B 12 concentrations detected in Bacillus megaterium ATCC 14581 cultures supplemented with 0, 1, or 10 μM CoCl2 after 48 h ( n = 10).

Techniques: Control, Positive Control, Comparison, Derivative Assay